ABSTRACT
OBJECTIVE: To investigate the effect of LM49(2,4′, 5′-trihydroxy-5,2′-dibromobenzophenone) on M1/M2 phenotype in RAW264.7 macrophages by LPS plus INF-γ and its regulation mechanism on M1/M2 polarization. METHODS MTT assay was used to determine the effect of LM49 on cell viability. Different concentrations of LM49 (5, 10, 20 μmol•L-1) were used to intervene in LPS combined with IFN-γ induced macrophages, the expression of macrophage subsets of markers and the effect on JAK/STAT of the signaling pathway were examined by flow cytometry, RT-PCR and Western-blot. RESULTS: Compared with LPS/INF-γ group, LM49 significantly inhibited the number of CD16/32+ cells and the mRNA expression of iNOS, IL-6 and TNF-α, increased the number of CD206+ cells and the mRNA expression of Arg-1 and IL-10, and decreased the ratio of M1/M2 in macrophage. It was demonstrated that LM49 significantly reduced the proteins expression levels of TLR4, Myd88, NF-κB and STAT1, while inhibiting the phosphorylation levels of p-JAK2 and p-STAT1 by Western-blot. CONCLUSION: The study demonstrates that LM49 inhibits M1 polarization and promotes M2 polarization in RAW264.7 macrophages via inhibiting TLR4-Myd88-NF-κB and JAK2-STAT1 pathway, regulating the balance of M1/M2 ratio in macrophages.
ABSTRACT
OBJECTIVE: To study the characteristics and clinical application of radical laparoscopic myomectomy.METHODS: Retrospectively analyze the clinical data of 31 patients with uterine leiomyomas undergoing laparoscopic radical myomectomy at the Department of Obstetrics and Gynecology,the First Affiliated Hospital of Soochow University,from January 2016 to December 2018.The surgical indications,operation time,intraoperative blood loss,postoperative menstrual recovery,postoperative uterine fibroid recurrence rate and postoperative sexual and life quality change indicators were analyzed.RESULTS: All patients were with multiple uterine fibroids(averaged 42.6±3.5 years),and all of them received successful operation.There were no patients who underwent laparotomy or hysterectomy.The postoperative fibroids residual rate among the 31 patients was 3.23%(1 case).The recurrence rate of postoperative uterine fibroids half a year later was 9.68%(3 cases).Five patients has no significant change in postoperative menstruation volume while the other 25 patients had volume reduction(t=8.614,P0.05).Only one patient suffered from hot flashes,night sweating and other menopausal symptoms.WHOQOL-BREF scale was used for patients to assess preoperative and postoperative quality of life,and there were obviously improved psychological and physiological score in patients after operation(P<0.05).CONCLUSION: For women with multiple uterine fibroids who have surgical indications and a strong desire to retain the uterine but have no fertility requirements,radical laparoscopic myomectomy may be the most effective surgical therapy,which can achieve the purpose of surgical treatment,improving the resection rate,and maintaining low postoperative recurrence rate,preserving menstruation and the function of uterus and ovary,and increasing the sexual life quality.